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cDNA-AFLP analysis of Psidium guajava L. cultivars under water stress and mechanical injury: methodological implications Braz. J. Plant Physiol.
Furlan,Cláudia Maria; Zanotta,Samantha; Salatino,Antonio.
Studies involving amplified fragment length polymorphism (cDNA-AFLP) have often used polyacrylamide gels with radiolabeled primers in order to establish best primer combinations, to analyze, and to recover transcript-derived fragments. Use of automatic sequencer to establish best primer combinations is convenient, because it saves time, reduces costs and risks of contamination with radioactive material and acrylamide, and allows objective band-matching and more precise evaluation of transcript-derived fragments intensities. This study aimed at examining the gene expression of commercial cultivars of P. guajava subjected to water and mechanical injury stresses, combining analyses by automatic sequencer and fluorescent kits for polyacrylamide gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Automatic sequencer; Gene expression; Mechanical injury; Water stress.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000100005
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Post-harvest quality of fresh-marketed tomatoes as a function of harvest periods Scientia Agricola
Ferreira,Marcos David; Franco,André Toshiro Oshima; Kasper,Ricardo Freitas; Ferraz,Antonio Carlos Oliveira; Honório,Sylvio Luiz; Tavares,Marcelo.
Losses on tomato business chain start at harvest, a two-months period. At the beginning of the harvest, fruits concentrate at the basal part of the plant, then in the middle, and finally at the top, and undergo changes in diameter and maturity indexes as harvest progresses. The aim of this work was to evaluate the impact of handling at three different periods: (I) 15 days, (II) 30 days, and (III) 45 days after the beginning of harvest. Tomatoes were ordinarily grown and harvested in to bamboo baskets, and transferred to plastics boxes. Fruits were classified according to ripening stage and diameter, and evaluated for mechanical damage and external defects caused by harvesting procedures. The time required for the harvest operation was measured; damage to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Losses; Post-harvest; Mechanical injury.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000500006
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